Mapping molecular assemblies with fluorescence microscopy and object-based spatial statistics
نویسندگان
چکیده
منابع مشابه
Measurement of molecular diffusion based on optoelectrofluidic fluorescence microscopy.
This technical note reports a method for measuring the diffusion coefficient of molecules based on an optoelectrofluidic platform. Optoelectrofluidic fluorescence microscopy, which is constructed with an optoelectrofluidic device and a conventional fluorescence microscope, is a useful tool for controlling and detecting local molecular concentration in a fluid with a single light source. When we...
متن کاملSpatial light modulators in fluorescence microscopy
Spatial light modulators (SLMs) are becoming increasingly important in optical microscopy. These reflective or transmissive devices, based on microdisplays using liquid crystal or micromirror array technology, are used to achieve a variety of useful effects and imaging modes. When placed in an image plane, SLMs can be used to create arbitrary, computer controlled masks. These masks can then be ...
متن کاملEfficient Object Localization and Tracking with Discrete Spatial Mapping
EFFICIENT OBJECT LOCALIZATION AND TRACKING WITH DISCRETE SPATIAL MAPPING by Yunhui Zheng Department of Electrical and Computer Engineering Duke University
متن کاملFluorescence and fluorescence microscopy
The concept of specific cellular antigen staining by use of an immunofluorescent technique was introduced by AH COONS and co-workers about 60 years ago. Fluorescent probes are efficient tools and enable the detection of particular components in complex structures of organs including live cells. Under the condition of specific molecular interactions, fluorochrome labeled ligands allow the select...
متن کاملAnalyzing Receptor Assemblies in the Cell Membrane Using Fluorescence Anisotropy Imaging with TIRF Microscopy
Signaling within and between animal cells is controlled by the many receptor proteins in their membrane. They variously operate as trans-membrane monomers and homo- or hetero-dimers, and may assemble with ion-channels: analyses thereof are needed in studies of receptor actions in tissue physiology and pathology. Interactions between membrane proteins are detectable when pre-labeled with fluorop...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Communications
سال: 2018
ISSN: 2041-1723
DOI: 10.1038/s41467-018-03053-x